Functional Analysis of the Lupinus luteus Cyclophilin Gene Promoter Region in Lotus japonicus

Functional analysis of promoter sequences is important to understand the regulation gene expression. This study aimed investigate region Lupinus luteus cytoplasmic cyclophilin (LlCyP; AF178458). After bioinformatic analysis, four deletion fragments were fused β-glucuronidase reporter gene. We used Lotus japonicus as a model plant. Agrobacterium rhizogenes transformation L. japonicus, only longest (−1055 bp ATG) supported expression in root nodule parenchyma. Putative cis-elements located between −1055 and −846 subjected site-directed mutagenesis. Mutations incorporated TGATT AGATT motifs (cytokinin response) abolished GUS nodules, but mutated AAAGAT motif (OSE, organ-specific element) still activated mainly cells surrounding vascular bundle. Promoter mutation experiments suggest that responsible for are spanning from bp. constructed fragment, which DNA sequence −822 −198 was removed (pCYPMG). The arranged pCYPMG supports parenchyma it lower than whole region. obtained results may be useful transgene determinate indeterminate nodules.